As part of HT sequencing, Genechron laboratory carries out transcriptomics projects through the use of RNA-Seq technique.
RNA-seq has revolutionized the exploration of gene expression and genome annotation. Advances in the sequencing techniques, from sample preparation through data analysis, enable rapid profiling and deep investigation of the transcriptome.
With RNA-seq, you can characterize all transcriptional activity, coding and non-coding, in any organism without any a priori assumptions. GAIIX Illumina system allows a unique combination of long and short reads, single and paired-end sequencing, and a capacity of tens of millions of reads per run which allows to

• annotate coding SNPs
• exome annotation of new genomes discovered
• discover transcript isoforms
• identify regulatory RNAs
• characterize splice junctions
• determine the relative abundance of transcripts

The delivered service offered by Genechron includes:

• New Truseq libraries Preparation
• Eventual sample Multiplexing
• Validation of the libraries using the Bioanalyzer System (Agilent) and by quantitative analysis performed in real time using the specific Library Quantification Kit (Kapabiosystems) on the 7900 HT Fast RT-PCR (Life Technologies) system
• Hybridization and amplification of libraries on Truseq flow cell (SR or PE) by the Cluster Station (Illumina)
• Validation of clusters amplified using GAIIx system (Illumina)
• SR or PE sequencing using GAIIx system (Illumina)
• Analysis of data quality and filtering of FASTQ sequences
• bioinformatics analysis (if required)