Next Generation (Next-Gen) sequencing refers to revolutionary new technology allowing low cost, high throughput sequencing for the production of millions of DNA sequence reads in a single run, thus providing an inexpensive, genome-wide sequence readout.

The Illumina Genome AnalyzerIIx uses sequencing by synthesis (SBS) chemistry to support massively parallel sequencing.
Based on novel, reversible, fluorescently-labeled terminators, this technology detects single bases as they are incorporated into growing DNA strands. Since all four reversible-terminator dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias. Homopolymers pose no problems as each cycle interrogates only one base at a time per template.

The Genome AnalyzerIIx system supports sequencing of both single-read and paired-end libraries. It is the only platform available that offers a short-insert paired-end capability for high-resolution sequencing as well as long-insert paired-end reads for efficient sequence assembly, de novo sequencing, and large-scale structural variation detection.
Illumina’s simple library construction protocol minimizes the time from sample isolation to usable results. Single-read or short-insert paired-end sample preparation of genomic DNA takes as few as six hours with only three hours of hands-on time. The combination of short inserts and 2 × 150 bp or longer reads increases the ability to align and sample the genome, expanding the Genome Analyzer’s utility for other applications.

The DNA preparation for sequencing includes random fragmentation, ligation of specific adaptors to these fragments and cluster generation. The Genome AnalyzerIIx system is available in our facility and Genechron can thus offer one of the best choices for De Novo Sequencing, Re-sequencing / Genotyping, Chip-Seq or Expression Profiling applications. Furthermore, the sequencing platform allows multiplexing of different samples in a single channel.

Illumina genome analysis scheme
(source: www.illumina.com)